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Current Protocols

Last Updated: Dec 14, 2023 4:24 PM

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Current Protocols in Bioinformatics features step-by-step peer-reviewed instructions for searching a diverse set of key bioinformatics databases and effectively using bioinformatics tools. Databases covered include the major multi-database sequence sites (NCBI and EBI), as well as specialized databases [WormBase, MINT (Molecular Interactions), KEGG (Kyoto Encyclopedia of Genes and Genomes), DrugBank, PharmGKB, ChemBank]. Topics include finding genes, RNA sequence analyses and predictions, sequence similarity and assembly tools, working with protein domains, building biological databases, analyzing expression patterns, and more. Updated every three months.

Designed for those studying the analysis of cell structures and functions, Current Protocols in Cell Biology contains peer-reviewed detailed protocols and related information which permit the analysis of cells at the molecular, single cell, or tissue level. Topics include motility, cell cycle, aging, or death, signal pathways, gel electrophoresis, microscopic techniques (confocal, fluorescence), signal transduction, differentiation pathways, antibodies as tools, whole organism or organelle methodologies, subcellular fractionation and more. Contains a set of appendices covering information on the use of inhibitors and pharmacologic agents, protein motifs, absorption and emission maxima for common fluorophores and PCR. Updated every three months.

A peer-reviewed "best-practices" collection that provides comprehensive coverage of both classic and emerging immunological methods.  Current Protocols in Immunology covers the latest in antibody detection and preparation, molecular immunology, innate immunity, tumor immunology, engineering immune molecules and receptors, detection and analysis of HIV, and isolation of stem and progenitor cells.  Offers five appendices, one devoted to the CD system of leukocyte surface molecules in human, mouse, and rat. Updated every three months.

The original Current Protocols title, Current Protocols in Molecular Biology provides peer-reviewed protocols and procedures ranging from basic methods for  cell culture or preparing/analyzing DNA, RNA, chromatin, glycoconjugates, and proteins, to advanced procedures including DNA sequencing, recombinant libraries, microarray analyses, mutagenesis, polymerase chain reaction (PCR), high-throughput sequencing, gene editing (CRISPR), combinatorial libraries, and gene silencing. Appendices include cloning vectors, commonly used reagents techniques, and pathway modulators/inhibitors. Updated every three months

Current Protocols in Neuroscience offers a search and retrieval interface to peer-reviewed core techniques considered to be the benchmark standard in neuroscience research. Brings together hundreds of core techniques in all branches of neuroscience, drawing from molecular neurobiology, neurophysiology, neuroanatomy, neuropharmacology, and behavioral neuroscience. Includes in vitro, in vivo and preclinical models. Methodologies range from imaging, neuroanatomical (histochemistry, metabolic mapping, fluorescence in situ hybridization), neural gene expression, neurotransmitters, to behavioral protocols in animal models. Appendices cover Internet resources and databases in general bioinformatics as well as neurogenetics. Updated every three months.

A comprehensive protein-centric resource, Current Protocols in Protein Science contains peer-reviewed methodologies for protein expression, characterization, and purification, post-translational modifications, identification and quantitation of interactions, proteomics, engineering, recombinant proteins, structural characterizations including protein folding, computational protein modeling, and techniques such as mass spectrometry and polymerase chain, and electrophoresis Updated every three months.

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Pamela Rose
Health Sciences Library
University at Buffalo
3435 Main St.
Buffalo, NY 14214